中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (3): 443-448.doi: 10.3969/j.issn.1673-8225.2012.03.014

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

骨髓间充质干细胞复合小肠黏膜下层构建组织工程皮肤修复糖尿病      
皮肤缺损*☆

王少云1，吴  迪2，张  丽3，朱晓松2，杨  浩2，李次会4，武  术4，李世和2，董  坚5   

  1. 1昆明医学院第三附属医院骨科，云南省昆明市   650000；2昆明医学院第一附属医院骨科，云南省昆明市   650032；3昆明医学院基础医学院机能教研室，云南省昆明市   650031；4北京大清生物公司，北京市   100000；5昆明医学院第一附属医院生物治疗中心，云南省昆明市   650032
  • 收稿日期:2011-05-21 修回日期:2011-06-16 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 通讯作者：董坚，博士，博士生导师，昆明医学院第一附属医院生物治疗中心，云南省昆明市 650032
  • 作者简介:王少云☆，男，1982年生，云南省昆明市人，白族，2010年昆明医学院毕业，博士，医师，主要从事骨科创伤、骨肿瘤临床及科研工作研究。Reinhard007550@163.com
  • 基金资助:

    本课题受云南省科技厅-昆明医学院联合资金资助(2007C0002R)。

Bone marrow mesenchymal stem cells combined with small intestinal submucosa to construct tissue-engineered skin for repair of diabetes mellitus induced full-thickness skin defects

Wang Shao-yun1, Wu Di2, Zhang Li3, Zhu Xiao-song2, Yang Hao2, Li Ci-hui4, Wu Shu4, Li Shi-he2, Dong Jian5   

  1. 1Department of Orthopedics, Third Affiliated Hospital, Kunming Medical University, Kunming 650000, Yunnan Province, China; 2Department of Orthopedics, First Affiliated Hospital, Kunming Medical University, Kunming  650032, Yunnan Province, China; 3Function Department, Basic Medical School of Kunming Medical University, Kunming  650031, Yunnan Province, China; 4Beijing Datsing Bio-Tech, Beijing 100000, China; 5Centre of Biotherapy, First Affiliated Hospital, Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2011-05-21 Revised:2011-06-16 Online:2012-01-15 Published:2012-01-15
  • Contact: Dong Jian, Doctor, Doctoral supervisor, Centre of Biotherapy, First Affiliated Hospital, Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Wang Shao-yun☆, Doctor, Physician, Department of Orthopedics, Third Affiliated Hospital, Kunming Medical University, Kunming 650000, Yunnan Province, China Reinhard007550@ 163.com
  • Supported by:

    United Foundation of Yunnan Science and Technology Bureau & Kunming Medical University, No. 2007C0002R*

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摘要：

背景：小肠黏膜下层已经被证实可以引导许多组织的特异性再生。
目的：将骨髓间充质干细胞复合猪小肠黏膜下层构建组织工程皮肤并移植修复兔糖尿病皮肤缺损，观察其对糖尿病皮肤缺损的愈合影响和疗效。
方法：制作兔糖尿病皮肤缺损，将骨髓间充质干细胞与冻干、灭菌、水合后的猪小肠黏膜下层膜复合制作组织工程皮肤并覆盖修复兔糖尿病皮肤缺损，并与小肠黏膜下层支架组和空白对照组做对照。
结果与结论：造模后1，2，4周，组织工程皮肤组缺损皮肤修复面积百分比显著大于小肠黏膜下层支架及空白对照组(P < 0.05)。缺损皮肤修复组织观察组织工程皮肤组组织生长明显快于小肠黏膜下层组与空白对照组。3组均未见明显皮肤附件结构。表明骨髓间充质干细胞复合猪小肠黏膜下层构建的组织工程皮肤修复兔糖尿病皮肤缺损好于单纯小肠黏膜下层膜，无明显瘢痕组织的生成，可作为支架材料用于皮肤缺损的修复。

关键词: 组织工程皮肤, 糖尿病, 骨髓间充质干细胞, 猪小肠粘膜下层, 皮肤缺损

Abstract:

BACKGROUND: Small intestinal submucosa (SIS) has been shown to guide the specific regeneration of many tissues.
OBJECTIVE: To construct tissue-engineered skin by bone marrow mesenchymal stem cells (BMSCs) combined with SIS to treat full-thickness skin defects in rabbits with diabetes mellitus, and to observe the healing condition and effects.
METHODS: Full-thickness skin defect models were prepared in rabbits with diabetes mellitus. BMSCs with SIS were used to construct tissue-engineered skin for full-thickness skin defects in comparison with SIS scaffold and blank control group.
RESULTS AND CONCLUSION: The repairing area in the combination group was larger than that of SIS scaffold and blank control groups (P < 0.05). The rate of skin growth in the combination group was also faster than that of the other two groups. There were no skin appendage structures in the three groups. It was indicated that the satisfactory effect was obtained by tissue engineered skin transplantation, and the tissue engineered skin could be used to treat the full-thickness skin defects following diabetes mellitus.

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